| Guinness Premiership |
| Sat 05 Sept 2009 | Wasps - Harlequins | 26-15 |
|
| Sat 12 Sept 2009 | Harlequins - Leicester | (B) 9-15 |
|
| Sat 19 Sept 2009 | Harlequins - Saracens | 9-22 |
|
| Fri 25 Sept 2009 | Newcastle - Harlequins | 17-17 |
|
| Sat 03 Oct 2009 | Harlequins - Bath | 13-11 |
|
| Sat 24 Oct 2009 | Worcester - Harlequins | 22-26 |
|
| Sat 31 Oct 2009 | Harlequins - London Irish | 9-9 |
|
| Sat 21 Nov 2009 | Northampton - Harlequins | 26-17 |
|
| Sat 28 Nov 2009 | Harlequins - Gloucester | (B) 35-29 |
|
| Fri 04 Dec 2009 | Leeds - Harlequins | 27-30 |
|
| Sun 27 Dec 2009 | Harlequins - Wasps | (B) 20-21 |
|
| Fri 01 Jan 2010 | Sale - Harlequins | 21-16 (B) |
|
| Sat 09 Jan 2010 | Harlequins - Leeds | Reported |
|
| Sat 13 Feb 2010 | Gloucester - Harlequins | 46-6 |
|
| Sat 20 Feb 2010 | Harlequins - Northampton | 13-6 |
|
| Sun 28 Feb 2010 | London Irish - Harlequins | 29-14 |
|
| Sat 06 Mar 2010 | Harlequins - Worcester | 14-11 |
|
| Sat 27 Mar 2010 | Bath - Harlequins | |
|
| Sat 03 Apr 2010 | Harlequins - Newcastle | |
|
| Sun 18 Apr 2010 | Saracens - Harlequins | |
|
| Sat 24 Apr 2010 | Leicester - Harlequins | |
|
| Sat 08 May 2010 | Harlequins - Sale | |
|
